Rabbit anti-GAT-2 (Gamma Aminobutyric Acid Transporter) Antibody

Name

Rabbit anti-GAT-2 (Gamma Aminobutyric Acid Transporter) Antibody

中文名字

兔抗GAT-2抗体；兔抗γ-氨基丁酸转运蛋白抗体

Description

The GAT-2 antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat retina and leptomeninges using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/500 – 1/1,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. The antiserum has been characterized as specific to GAT-2; please see references listed below. GAT-2 immunolabeling is completely abolished by soluble pre-adsorption with synthetic rat GAT-2 (594-602) at a concentration of 10-5 M.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

Sodium- and chloride-dependent GABA transporter 2; Solute carrier family 6 member 13; Gabt2; solute carrier family 6 (neurotransmitter transporter, GABA), member 13, anti-GAT2

RRID

AB_572237

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Slc6a13

Entrez Gene ID

Entrez Gene:  171163    Rat

NCBI Gene Aliases

/

Sequence

Q9NSD5

APPLICATION

Quality Control

The GAT-2 antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat retina and leptomeninges using indirect immunofluorescent and biotin/avidin-HRP techniques. The antiserum has been characterized as specific to GAT-2. GAT-2 immunolabeling is completely abolished by soluble preadsorption with synthetic rat GAT-2 (594–602) at a concentration of 10–5 M.

Tissue

Rat leptomeninges and retina

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
 • Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/AV-HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/500–1/2,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.