Rabbit anti-GHRF (Growth Hormone Releasing Factor) Antibody

Name

Rabbit anti-GHRF (Growth Hormone Releasing Factor) Antibody

中文名字

兔抗生长激素释放因子抗体；兔抗GHRF抗体

Description

The antibody produces strong labeling of GHRH at a dilution of 1/2,000 – 1/4,000 using biotin/streptavidin HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling tehcnique and/or detection system; therefore, a dilution series is recommended.
Cross reactivity of GHRF antiserum was examined using the paper spot technique of Larsson (1981). Using 2 µL, 100 pmole amounts, the following substances did not react with rat GHRF antisera diluted 1/500 using the PAP labeling method: glucagon, gastric inhibitory peptide, secretin, vasoactive intestinal peptide, peptide histidine isoleucine, pancreatic polypeptide (human or rat), human GHRF, somatostatin, insulin, ACTH, motilin, cholecystokinin octapeptide, substance P, molluscan cardioexcitatiory peptide, gastrin 34, and serotonin. GHRF antiserum had a very good reactivity using rat GHRF at 2 µL, 100 pmole amounts.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

GRF; GHRH; Somatoliberin; Growth hormone-releasing hormone; Sermorelin; Somatocrinin; Somatorelin; Ghrf; growth hormone releasing hormone, anti-GHRF

RRID

AB_572239

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Ghrh

Entrez Gene ID

Entrez Gene: 29446  Rat

NCBI Gene Aliases

/

Sequence

P01286, 9034-39-3;HO - Tyr - Ala - Asp - Ala - Ile - Phe - Thr - Asn - Ser - Tyr - Arg - Lys - Val - Leu - Gly - Gln - Leu - Ser - Ala - Arg - Lys - Leu - Leu - Gln - Asp - Ile - Met - Ser - Arg - Gln - Gln - Gly - Glu - Ser - Asn - Gln - Glu - Arg - Gly - Ala - Arg - Ala - Arg - Leu - NH2

APPLICATION

Quality Control

The antibody produces significant labeling of GHRH at dilutions of 1/1,000–1/5,000 using biotin/streptavidin HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Cross reactivity of GHRF antiserum was examined using the paper spot technique of Larsson (1981). Using 2 µL, 100 pmole amounts, the following substances did not react with rat GHRF antisera diluted 1/500 using the PAP labeling method: glucagon, gastric inhibitory peptide, secretin, vasoactive intestinal peptide, peptide histidine isoleucine, pancreatic polypeptide (human or rat), human GHRF, somatostatin, insulin, ACTH, motilin, cholecystokinin octapeptide, substance P, molluscan cardioexcitatory peptide, gastrin 34, and serotonin. GHRF antiserum had a very good reactivity using rat GHRF at 2 µL, 100 pmole amounts.

Tissue

Rat hypothalamus (median eminence)

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use IF or Bn-SA/HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/1,000–1/5,000 in PBS/0.3% Triton X-100 – Bn-SA/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.