Cat. No | MBA-029 |
Name | Rabbit anti-Glucagon Antibody |
中文名字 | 兔抗胰高血糖素抗体 |
Description | The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Chick, Human, Mouse, Pig, Rat, Sea Lamprey, Snail, Three-Spined Stickleback (Fish), Turtle |
Preabsorption Control | / |
Alternate Names | anti-glucagon |
RRID | AB_572241 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | GCG |
Entrez Gene ID | Entrez Gene: 396196 Chick |
NCBI Gene Aliases | / |
Sequence | P01275; HSQGTFTSDYSKYLDSRRAQDFVQWLMNT |
APPLICATION | |
Quality Control | The antibody has significant Biotin/avidin-HRP immunostaining at a 1/500–1/1,000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon. Preadsorption of the diluted antibody with an excess of the following substances had no effect on glucagon labeling: secretin, vasoactive intestinal peptide, peptide histidine isoleucine-27, gastric inhibitory polypeptide, rat and human growth hormone releasing hormone and somatostatin. |
Tissue | Human Pancreatic Islets |
Perfusion Fixation | Formalin |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 0.5 hour at Room Temperature |
Detection System | Use Bn/AV-HRP reagents at dilutions recommended by the manufacturer. |
Suggested Dilution | 1/500–1/1,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles. |
