Rabbit anti-Glucagon-like Protein Receptor (GLP2R) Antibody

Name

Rabbit anti-Glucagon-like Protein Receptor (GLP2R) Antibody

中文名字

兔抗胰高血糖素样蛋白受体（GLP2R）抗体；兔抗GLP2R抗体

Description

The Rabbit antibody for Glucagon-like Protein Receptor is generated for acetyl 65-88 amide sequence targeting rat and human proteins, but not mouse. The peptide was synthesized and cross-linked to keyhole limpet hemocyanin via sulfolink coupling.  The antibody is provided as 100 µL of affinity purified serum containing 1% BSA.
Produced by Dr. Mark Brownfield, the peptide sequence encoding the rat GLP2R was retrieved from the NCBI protein database and evaluated using GeneRunner software to generate antigen candidates for antibody production.  Antibody was generated in rabbits and purified by affinity chromatography against the antigenic peptide. The specificity of the antibody was confirmed by Western blotting and by immunoabsorption controls is the immunohistochemistry procedure (see Nelson et al, Endocrinology 148(5)1954-1962, 2007.
The antiserum demonstrates significant labeling of enteroendocrine cells in the intestinal epithelium, as well as cell bodies of vagal afferents in nodose ganglia of the parasympathetic nervous system. Immunolabeling of Western blot revealed a band of approximately 66 kDa in human and rat tissue. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

anti-GLP2R

RRID

to be determined

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Entrez Gene ID

Entrez Gene: 50592       Rat

NCBI Gene Aliases

/

Sequence

O95838

APPLICATION

Quality Control

The GLP2 receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of enteroendocrine cells in the intestinal epithelium, as well as cell bodies of vagal afferents in nodose ganglia of the parasympathetic nervous system. Labeling is effective using indirect immunofluorescent and biotin/avidin-HRP techniques. The addition of intensifying reagents such as tyramide amplification or nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling of western blot revealed a band of approximately 66 kDa in human and rat tissue, but not mouse. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.

Tissue

Rat and Human intestinal epithelium and nodose ganglia.

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

Sections

50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C

Detection System

Use Bn/Av-HRP at dilutions recommended by the manufacturer

Suggested Dilution

1/800-1/1000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2 º–8ºC until expiration date.