Rabbit anti-Histamine Antibody

Name

Rabbit anti-Histamine Antibody

中文名字

兔抗组胺抗体

Description

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain.
The Histamine antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 biotin/avidin-HRP Technique . All staining is blocked by preabsorption of the antiserum with Histamine conjugate. Cross reactivity experiments indicate no cross reactivity with L-histidine or L-histidine containing peptides such as LH-RH.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Aplysia Californica (Sea Slug), Bee, Copepods (Crustacean), Crab, Drosophila (Fly), Drosophila Melanogaster (Fly), Flea, Fly, Fruit Fly, Locust, Marine, Monkey, Moth, Mouse, Pig, Pleurobranchaea Japonica (Sea Slug), Rat, Snail

Preabsorption Control

/

Alternate Names

anti-Histamine

RRID

AB_572245

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Entrez Gene ID

NCBI Gene Aliases

/

Sequence

51-45-6

APPLICATION

Quality Control

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. In the central nervous system, histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain. In rat hypothalamus the antiserum has significant staining using the indirect immunofluorescence method and the biotin-streptavidin/HRP staining method at a 1/4,000–1/6,000 dilution. All staining is blocked by preabsorption of the antiserum with Histamine conjugate. Cross reactivity experiments indicate no cross reactivity with L-histidine or L-histidine containing peptides such as LH-RH.

Tissue

Rat Hypothalamus

Perfusion Fixation

• Fixation: 4% carbodiimide in phosphate buffered saline, pH 7.4; 200 mL over 20 min.
• Post Fixation: 1.5 hr. at 4°C in 4% carbodiimide in phosphate buffered saline, pH 7.4.

Absorption Control

Sections

10 µm cryostat (vibratome sections are not recommended)

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/AV-HRP according to manufacturer’s directions.

Suggested Dilution

1/4,000–1/6,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.