Cat. No | MBA-033 |
Name | Rabbit anti-Insulin Antibody |
中文名字 | 兔抗胰岛素抗体 |
Description | The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep |
Preabsorption Control | / |
Alternate Names | anti-Insulin |
RRID | AB_572246 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | INS |
Entrez Gene ID | Entrez Gene: 102396436 Bubalus Bubalis (Buffalo) |
NCBI Gene Aliases | / |
Sequence | P01308 |
APPLICATION | |
Quality Control | The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat pancreatic beta cells using indirect immunofluorescence and biotin/avidin-HRP techniques. Immuostaining is completely abolished by soluble preadsorption with bovine insulin at 10 µg per mL of diluted antiserum. |
Tissue | Rat Pancreas (Pancreatic islets) |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20-30 min. |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn/AV-HRP reagents at dilutions recommended by the manufacturer. |
Suggested Dilution | 1/3,000–1/5,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º-8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15ºC or lower. Avoid repeated freeze/thaw cycles. |
