Cat. No | MBA-034 |
Name | Rabbit anti-Leucine Enkephalin Antibody |
中文名字 | 兔抗亮氨酸脑啡肽抗体 |
Description | The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat globus pallidus and spinal cord. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Bird, Bovine, Bullfrog, Cat, Chick, Dog, Fish, Frog, Guinea Pig, Hamster, Human, Lungfish, Monkey, Mouse, Octopus, Pig, Pigeon, Rat, Ray, Shark, Sheep, Starling, Three-Spined Stickleback, Turtle, Zebra Finch |
Preabsorption Control | / |
Alternate Names | Enkephalin (Leu+Met); Proenkephalin-A; enkephalin A; preproenkephalin, anti-Leucine Enkephaline, Leu Enk |
RRID | AB_572247 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | PENK |
Entrez Gene ID | Entrez Gene: 281387 Bovine |
NCBI Gene Aliases | / |
Sequence | 58822-25-6 |
APPLICATION | |
Quality Control | The antibody produces significant indirect immunofluorescent staining and significant biotin-avidin/HRP staining at a 1/1,000–1/2,000 dilution in rat globus pallidus and spinal cord. Staining is completely eliminated by pretreatment with 50 µg of Leucine Enkephalin per mL of diluted antiserum. Pretreatment with 50 µg of Methionine Enkephalin per mL of diluted antiserum also significantly blocks staining. |
Tissue | Rat globus pallidus and spinal cord |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1 M Phosphate Buffer, pH 7.4; 500 mL over 20–30 min. |
Absorption Control | Leu-Enk 50 µg/mL diluted serum |
Sections | 10 µm cryostat or 50 µm vibratome |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn/AV-HRP reagents at dilutions recommended by the manufacturers. |
Suggested Dilution | 1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn-Av/HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate a 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles. |
