Rabbit anti-LHRH (Luteinizing Hormone Releasing Hormone) / GnRH Antibody

Name

Rabbit anti-LHRH (Luteinizing Hormone Releasing Hormone) / GnRH Antibody

中文名字

兔抗促黄体生成素释放激素/促性腺激素释放激素抗体；兔抗LHRH/GnRH抗体

Description

The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 – 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection.
Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

P01148

Preabsorption Control

/

Alternate Names

Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH

RRID

AB_572248

Immunogen

This information is proprietary to Mabioway

Gene Symbol

GNRH1

Entrez Gene ID

Entrez Gene:103295055    Bat
Entrez Gene: 770134        Chick
Entrez Gene: 100135026   Frog
Entrez Gene: 100135531   Guinea Pig
Entrez Gene: 100774729    Hamster
Entrez Gene: 2796             Human
Entrez Gene: 101705387    Mole Rat
Entrez Gene: 613033         Monkey
Entrez Gene: 14714           Mouse
Entrez Gene: 397516         Pig
Entrez Gene: 25194           Rat
Entrez Gene: 100135917    Trout

NCBI Gene Aliases

/

Sequence

P01148

APPLICATION

Quality Control

The antibody produces significant labeling of LHRH at dilutions of 1/2,000–1/4,000 using indirect immunofluorescence and using biotin-avidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.

Tissue

Rat hypothalamus (median eminence)

Perfusion Fixation

• Fixation: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjugation with paraformaldehyde.

Absorption Control

LHRH 5 µg/mL diluted serum

Sections

10 µm cryostat or 50 µm vibratome.

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use IF or Bn-AV/HRP reagents at dilutions recommended by the manufacturers

Suggested Dilution

1/2,000–1/4,000 in PBS/0.3% Triton X-100 - Bn-AV/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles