Rabbit anti-Methionine Enkephalin Antibody

Name

Rabbit anti-Methionine Enkephalin Antibody

中文名字

兔抗甲硫氨酸脑啡肽抗体

Description

The antibody has a proven strong biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala.
Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Astyanax Jordani (Fish), Bird, Blowfly, Bovine, Bullfrog, Cat, Chick, Chicken, Cockroach, Cod, Crab, Dog, Fish, Frog, Gerbil, Goldfish, Guinea Pig, Hamster, Human, Lamprey, Leech, Lymnaea Stagnalis (Snail), Manducta Sexta (Moth), Monkey, Mouse, Octopus, Parrot, Pig, Pigeon, Rabbit, Rana Esculenta (Frog), Rana Pipiens (Leopard Frog), Rana Temporaria (Frog), Rat, Ratfish, Salamander, Salmon, Sea Bass (Fish), Shrew, Squirrel Monkey, Starling, Tapeworm, Turtle, Zebra Finch

Preabsorption Control

/

Alternate Names

Enkephalin (Met); Proenkephalin-A; Met-enkephalin; Opioid growth factor; OGF, anti-Methionine Enkephalin, Met Enk

RRID

AB_572250

Immunogen

This information is proprietary to Mabioway

Gene Symbol

PENK

Entrez Gene ID

Entrez Gene: 281387        Bovine
Entrez Gene: 768277        Cat
Entrez Gene: 421131        Chick
Entrez Gene: 100687307   Dog
Entrez Gene: 548461        Frog
Entrez Gene: 100379621   Guinea Pig
Entrez Gene: 101844627   Hamster
Entrez Gene: 5179            Human
Entrez Gene: 696649        Monkey
Entrez Gene: 18619          Mouse
Entrez Gene: 100152093   Pig
Entrez Gene: 102088743   Pidgeon
Entrez Gene: 100342780   Rabbit
Entrez Gene: 29237          Rat
Entrez Gene: 100196814   Salmon
Entrez Gene: 101552431   Shrew
Entrez Gene: 106851221   Starling
Entrez Gene: 102933698   Turtle
Entrez Gene: 100190068   Zebra Finch

NCBI Gene Aliases

/

Sequence

58569-55-4

APPLICATION

Quality Control

The antibody produces significant indirect immunofluorescent staining at a 1/400–1/600 dilution and significant biotin-avidin/HRP staining at 1/2,000 dilution in rat globus pallidus and amygdala. Staining is completely eliminated by pretreatment with 5 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 5 µg of leucine enkephalin per mL of diluted antiserum only partially blocks staining, and as leucine enkephalin concentration goes up, more diluted antiserum is blocked.

Tissue

Rat globus pallidus and amygdala

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 ml over 20–30 min.
• Post Fixation: 2 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer pH 7.4.

Absorption Control

Met Enk 5 µg/mL diluted antiserum

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by the manufacturers.

Suggested Dilution

1/2,000–1/5,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.