Rabbit anti-Neurokinin 1 Receptor Antibody

Name

Rabbit anti-Neurokinin 1 Receptor Antibody

中文名字

兔抗神经激肽1受体抗体

Description

The NK1R antiserum was quality control tested using standard immunohistochemical methods in rat brain using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 10 mg of rat NK1R peptide residues 393-407.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

Tachykinin receptor 1; NK-1 receptor; SPR, Substance-P receptor; tachykinin peptide hormone; Tacr1r, anti-NK-1

RRID

AB_10013880

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Tacr1

Entrez Gene ID

Entrez Gene: 24807   Rat

NCBI Gene Aliases

/

Sequence

P25103

APPLICATION

Quality Control

The NK1R antiserum was quality control tested using standard immunohistochemical methods in rat brain using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble preadsorption and western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK1R peptide residues (393–407). Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 70 and 110 kD.

Tissue

Rat brain - striatum and cortex

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component in fixation. If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Rat NK1R (393–407) 10 ug/mL diluted antibody completely eliminates immunolabeling

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

48 hours at 2°–8°C

Detection System

Bn/AV-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/3,000–1/5,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that users perform a primary antibody dilution series using the dilution
recommendations above as a guideline. Note that a change in the fixation or buffering system from our
protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2°–8°C up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C. or lower. Avoid repeated freeze/thaw cycles.