Rabbit anti- nNOS antibody（C-Terminal）

Name

Rabbit anti- nNOS antibody（C-Terminal）

中文名字

兔抗nNOS抗体；兔抗神经元一氧化氮合酶

Description

The neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/8,000 – 1/12,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Cat, Chick, Ferret, Guinea Pig, Hamster, Human, Monkey, Mouse, Opossum, Rat, Shrew, Squirrel Monkey, Vole, Zebrafish

Preabsorption Control

/

Alternate Names

Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNos:C-Terminal

RRID

AB_572256

Immunogen

This information is proprietary to Mabioway

Gene Symbol

NOS1

Entrez Gene ID

Entrez Gene: 101100342    Cat
Entrez Gene: 427721         Chick
Entrez Gene: 101692439    Ferret
Entrez Gene: 100729682    Guinea Pig
Entrez Gene: 100758127    Hamster
Entrez Gene: 4842             Human
Entrez Gene: 693781          Monkey
Entrez Gene: 18125            Mouse
Entrez Gene: 100013712     Opossum
Entrez Gene: 24598            Rat
Entrez Gene: 102850179     Shrew
Entrez Gene: 101034544     Squirrel Monkey
Entrez Gene: 101984335     Vole
Entrez Gene: 60658            Zebrafish

NCBI Gene Aliases

/

Sequence

P29475

APPLICATION

Quality Control

The neuronal Nitric Oxide Synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Western blot analysis of brain homogenates shows the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by preadsorption with synthetic human nNOS (1419–1433) at 5 mg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed.

Tissue

Rat hypothalamus, striatum, cortex and spinal cord

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

 Use Cy3 or Bn/Av-HRP reagents at dilutions recommended by the manufacturers.

Suggested Dilution

 1/8,000–1/12,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles