Rabbit anti-nNOS antibody（N-Terminal）

Name

Rabbit anti-nNOS antibody（N-Terminal）

中文名字

兔抗nNOS抗体；兔抗神经元一氧化氮合酶

Description

The N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Mouse, Rat

Preabsorption Control

/

Alternate Names

Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNOS:N-Terminal

RRID

AB_572255

Immunogen

This information is proprietary to Mabioway

Gene Symbol

NOS1, 4842

Entrez Gene ID

NCBI Gene Aliases

/

Sequence

P29475

APPLICATION

Quality Control

The N-terminal neuronal Nitric Oxide Synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. By western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by preadsorption with synthetic human nNOS (134-148) at 5 mg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.

Tissue

Rat hypothalamus, striatum, cortex and spinal cord

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/Av-HRP reagents as instructed by the manufacturer.

Suggested Dilution

1/10,000–1/15,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. Please read the instruction booklet carefully before beginning the procedure.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles