Name
LDHB
Cat. No.
MAG-3061
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
At Room Temperature
Description
The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
Introduction
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Purification Method
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Physical Appearance
Sterile lyophilized powder.
Formulation
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Stability
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The specific activity was found to be 258 U/mg protein.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Lactate Dehydrogenase, LDH.
Amino acid sequence
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Background
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