Name
UNG E.Coli
Cat. No.
MAG-3194
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
UNG E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-229 a.a) and having a molecular mass of 28.1kDa. UNG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
UNG is a member of the Uracil-DNA glycosylase family. One of his functions is to prevent
mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and
initiating the base-excision repair (BER) pathway. Uracil bases are formed as a result of cytosine
deamination or misincorporation of dUMP residues. After a mutation is formed,
the mutagenic threat
of uracil propagates through any subsequent DNA replication steps . Among the diseases associated with UNG are: congenital rubella,
and immunodeficiency with hyper igm type 4.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
UNG protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
UDG, b2580, JW2564, EC 3.2.2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2.2, HIGM4, UNG2.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA
FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ
GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN
QWLEQRGETP IDWMPVLPAE SE
Background
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