Name
TEV
Cat. No.
MAG-3676
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly.
Introduction
TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
Purification Method
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Physical Appearance
Sterile liquid formulation.
Formulation
The rTEV contains 25mM Tris, Ph 8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.
Stability
rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
rTEV, TEV, P1 protease.
Amino acid sequence
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Background
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