Cat. No.
MAK-0029
Product Name
Guinea Pig Anti-KLH IgG
Storage
2-8°C
Species
Guinea Pig
Instructions
This kit is intended for measurement of anti-KLH IgG levels in guinea pig serum or plasma obtained 21 days after immunization with KLH.
Introduction
Measurement of KLH (keyhole limpet hemocyanin) induced antiKLH antibody levels allows quantitative evaluation of the immune response. This ELISA is designed for the rapid and quantitative measurement of guinea pig anti-KLH IgG levels in serum or plasma.
Principle Of The Assay
The guinea pig anti-KLH IgG test kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses KLH for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-guinea pig IgG (Fc specific) for detection. Serum or plasma samples are diluted and incubated in the microtiter wells for 1 hour. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 30 minutes. Anti-KLH IgG molecules are thus sandwiched between immobilized KLH and the detection antibody conjugate.
The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is
measured spectrophotometrically at 450 nm. The concentration of anti-KLH IgG is proportional to the optical density of the test sample.
