Cat. No.
MABL-020
Application
IP, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
P14
From
Recombinant Antibody
Specificity
The antibody binds to human NgR2 with a Kd of 2.3 nM and to rat NgR2 with a Kd of 0.48 nM (bivalent binding). The antibody binds specifically to residues 351-399 of NgR2, a member of the Nogo-66 receptor family which inhibits axonal growth and sporulating in the CNS.
Alternative Names
Nogo-66 Receptor
UniProt
Q86UN3
Immunogen
(His)6-tagged fragment of rat NgR2 (amino acids 279-420).
Application Notes
The original antibody was used for for an ELISA on the NgR2 antigen to confirm the binding of the antibody. This antibody was used for a western blot on lysates of 293F cells transfected with vectors for mammalian cell expression of human NgR1, NgR2, and NgR3. This antibody was used for flow cytometry on 293F cells transfected with human NgR1, NgR2, and NgR3 mammalian cell expression vectors. This antibody was used for surface plasmon resonance on NgR1 and NgR2 to determine its binding affinity for its antigen. The antibody was used for immunofluorescence on PC12 cells and PC12-NgR1 or PC12-NgR2. This antibody was used for immunoprecipitation on rat PC12 cell lines stably expressing full- length rat NgR1 or NgR2 (Hofer et al., 2008; PMID:17140598).
Antibody First Published
Hofer et al. Chimeric Rabbit/Human Fab and IgG Specific for Members of the Nogo-66 Receptor Family Selected for Species Cross-Reactivity with an improved Phage Display Vector.
Journal of Immunological Methods 2008; 318(1-2):75-87 PMID:17140598
Note on publication
Describes the generation of two monoclonal rabbit antibodies against members of the Nogo-66 receptor family.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
