Cat. No.
MABL-064
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
FR4D11
From
Recombinant Antibody
Specificity
FR4D11 antibody recognizes CD10. It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML).
Alternative Names
Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; EC 3.4.24; EC 3.4.24.11; FR4D11/F3
UniProt
P08473
Immunogen
This antibody was raised by immunizing mice with Raji cells.
Application Notes
FR4D11 antibody can be used to identify and analyse neprilysin (CD10) via various techniques. For instance, it was utilized in the localization of neprilysin in human cerebral cortex via immunohistochemistry (Akiyama et al., 2001). This antibody was also used during a FACS analysis and phenotype characterization of Breast Cancer Stem Cells (BrCSCs) (Petrelli et al., 2015).
Antibody First Published
Akiyama et al. Immunohistochemical localization of neprilysin in the human cerebral cortex: inverse association with vulnerability to amyloid beta-protein (Abeta) deposition. Brain Res. 2001 Jun 1;902(2):277-81. PMID:11384622
Note on publication
This article characterizes the FR4D11 antibody and uses it in the immunohistochemical investigation of neprilysin in the brain tissue.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
