Cat. No.
MABL-065
Application
IB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
D3/71
From
Recombinant Antibody
Specificity
This antibody was raised by immunising BALB/c mice with bacterially-expressed GST-MK1 fusion protein containing the Kv2.1 α subunit polypeptide.
Alternative Names
D3/71R; Kv2.1 voltage-gated potassium channel α subunit;Potassium voltage-gated channel subfamily B member 1; Kcnb1; Delayed rectifier potassium channel 1; DRK1; Voltage- gated potassium channel; Q03717; Q14721
UniProt
P15387
Immunogen
This antibody was raised by immunising BALB/c mice with bacterially-expressed GST-MK1 fusion protein containing the Kv2.1 α subunit polypeptide.
Application Notes
This antibody has been shown to immunolabel Kv2.1 expressed endogenously by neurons in mouse neocortex samples and COS-1 cells transfected to express rat Kv2.1 (Andrews et al, 2019). This antibody has also been used in immunoblot analysis of crude rat brain.
Antibody First Published
Bekele-Arcuri et al. Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides. Neuropharmacology.1996;35(7):851-65. PMID:8938716
Note on publication
Describes the original generation of this antibody, as part of a panel of anti-Kv2.1 monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
