Cat. No.
MABL-079
Application
IB, IP, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
K74/71
From
Recombinant Antibody
Specificity
This antibody is specific for Nav1.1. It does not cross-react with Nav1.2, Nav1.3 of Nav1.6.
Alternative Names
K74/71R; Voltage-gated sodium channel subunit alpha Nav1.1; Sodium channel protein type 1 subunit alpha; Sodium channel protein brain I subunit alpha; Scn1a; NAC1; SCN1
UniProt
P04774
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein consisting of the cytoplasmic C-terminus (amino acids 1929-2009) of rat Nav1.1.
Application Notes
The specificity of this antibody has been confirmed in ELISA against the GST-Nav1.1 fusion protein and immunofluorescence analysis of COS cells expressing full-length human Nav1.1 (Van Wart et al, 2005). This antibody has been used in immunoblot analysis of adult rat brain membranes, where it labels a single band at the same position as that labelled with pan-specific anti-Nav antibody K58/35 (Van Wart et al, 2005). Additionally, this antibody has been used in immunohistochemical analysis of adult rat hippocampus and whole brain sections (Van Wart et al, 2005; Andrews et al, 2019; PMID:30667360).
Antibody First Published
Van Wart et al. Novel clustering of sodium channel Nav1.1 with ankyrin-G and neurofascin at discrete sites in the inner plexiform layer of the retina Mol Cell Neurosci. 2005 Apr;28(4):661-73. PMID:15797713
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
