Cat. No.
MABL-114
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N206B/9
From
Recombinant Antibody
Specificity
This antibody is specific for GFAP and does not cross-react with GFAP-R416W or other proteins. GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative Names
N206B/9R; Glial fibrillary acidic protein; GFAP
UniProt
P14136
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.
Application Notes
This antibody is recommended for the detection and analysis of GFAP R416WT by western blot, immunocytochemistry and immunohistochemistry. For instance, the mouse version of this antibody was used to detect GFAP R416WT by western blot in brain samples from adult rat and GFAP wildtype, knockout and R416W KI mice. Futhermore it was used to detect GFAP R416WT by immunocytochemistry in GFAP WT and KO mouse hippocampus.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
