Cat. No.
MABL-115
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N212/17
From
Recombinant Antibody
Specificity
This antibody is specific for the constant region of TRIP8b, but it can cross react with several isoforms of TRIP8b. TRIP8b is an accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, regulating their cell-surface expression and cyclic nucleotide dependence.
Alternative Names
N212/17R; PEX5-related protein; PEX5-like protein; Peroxin-5-related protein; TPR-containing Rab8b-interacting protein; Tetratricopeptide repeat-containing Rab8b-interacting protein; Pex5Rp
UniProt
Q925N3
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b.
Application Notes
Immunohistochemistry was preformed on the brains of mice by using this antibody (Foote et al+AC53, 2019; PMID:31492750). The antibody was used for immunoprecipitation on HEK293T cells (Foote et al, 2019; PMID:31492750). This antibody was used for western blotting on proteins from rat brains (Zhou et al, 2015; PMID:25301101).AD53:AF53
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
