Cat. No.
MABL-124
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N277/7
From
Recombinant Antibody
Specificity
This antibody is specific for the synaptotagmin-12 protein. The antibody does not cross-react with Synaptotagmin-6 (or other Synaptotagmins). Synaptotagmin-12 is a synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release (By similarity). Unlike other synaptotagmins, it does not bind Ca2+ or phospholipids (By similarity).
Alternative Names
N277/7R; Syt12; Synaptotagmin XII; SytXII
UniProt
Q920N7
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 168- 255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12
Application Notes
This antibody is recommended for the detection and analysis of Synaptotagmin-12 by immunocytochemistry, immunohistochemistry and western blot. For instance, the mouse version of this antibody was used to detect Synaptotagmin-12 by western blot in extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin plasmid.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
