Cat. No.
MABL-137
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N348/82
From
Recombinant Antibody
Specificity
This antibody is specific for NSD3 epitope: amino acids 21-251 (N-terminus) of human NSD3. NSD3 preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Alternative Names
N348/82R; Histone-lysine N-methyltransferase NSD3; Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1
UniProt
Q9BZ95
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 21- 251 (N-terminus) of human NSD3
Application Notes
This antibody is recommended for detection and analysis of NSD3 by immunocytochemistry, immunohistochemistry and western blot. For instance the mouse version of this antibody was used to detect NSD3 by western blot in extracts of COS cells transiently transfected with HA- tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmid. Furthermore it was also used to detect NSD3 by immunohistochemistry in adult rat hippocampus.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
