Cat. No.
MABL-154
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N76/3
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 778-910 of HCN1. It shows no cross-reactivity against HCN2. HCN1 is a chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor.
Alternative Names
N76/3R; Atxn1; Spinocerebellar ataxia type 1 protein homolog; Ataxin-1
UniProt
P54254
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 778- 910 (cytoplasmic C-terminus) of rat HCN1.
Application Notes
This antibody has been used in western blot on the brain of a mouse (Hu et al, 2019; PMID:30649233). Immunohistrochemistry on the brain of a mouse has been preformed with this antibody (Hu et al, 2019; PMID:30649233). The antibody has shown use in immunofluorescence experiments on neurospheres, differentiates cells and cerebral tissues of mice (Edamakanti et al, 2018; PMID:29533923).
Antibody First Published
Edamakanti et al. Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1 J Clin Invest. 2018 Jun 1;128(6):2252-2265. PMID:29533923
Note on publication
Given the importance of this postnatal period for cerebellar development, it was asked whether this region might be developmentally altered by mutant ATXN1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
