Cat. No.
MABL-155
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N76/8
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 164-197 of HCN1. It shows no cross-reactivity against HCN2. HCN1 is a chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor.
Alternative Names
N76/8R; Atxn1; Spinocerebellar ataxia type 1 protein homolog; Ataxin-1
UniProt
P54254
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 164-197 of mouse ataxin-1.
Application Notes
This antibody has been used in a western blot experiment on mouse brain lysates and HeLa cell lysates (Suh et al, 2019; PMID:31442405) (Barclay et al, 2014; PMID:24179173).
Immunohistochemistry was preformed with this antibody on the brain of mice (Suh et al, 2019; PMID:31442405). The antibody has shown use immunoprecipitation experiments of HeLa cell lysate (Barclay et al, 2014; PMID:24179173).
Antibody First Published
Barclay et al. Systems biology analysis of Drosophila in vivo screen data elucidates core networks for DNA damage repair in SCA1 Hum Mol Genet. 2014 Mar 1;23(5):1345-64.
PMID:24179173
Note on publication
they performed a systematic in vivo screen of all available Drosophila melanogaster homolog DNA repair genes, and we tested the effect of their overexpression on lifespan and developmental viability in Spinocerebellar Ataxia Type 1 (SCA1) Drosophila models expressing human mutant Ataxin-1 (Atxn1).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
