Cat. No.
MABL-157
Application
ICC, KO, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
N84/37.1
From
Recombinant Antibody
Specificity
This antibody is specific for the human laforin protein. Laforin plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin.
Alternative Names
N84/37.1R; EPM2A; Glucan phosphatase; Glycogen phosphatase; Lafora PTPase; LAFPTPase
UniProt
O95278
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-331 (full-length) of human Laforin.
Application Notes
This antibody is recommended for detection and analysis of laforin by immunoblotting, immunocytochemistry, immunohistochemistry and knockout. For instance, the mouse version of this antibody was used to detect laforin by western blot in crude membrane fractions from whole rat or mouse brain and from human hippocampus, cerebral cortex or cerebellum.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
Describes the generation of a library of recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
