Cat. No.
MABL-159
Application
IP, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Aequorea victoria
Clone No.
N86/38.1
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 1-238 (full-length) of jellyfish green fluorescent protein. It cross-reacts with cyan and yellow fluorescent protein. It does not cross-react with red fluorescent protein. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
Alternative Names
N86/38.1R; Green fluorescent protein
UniProt
P42212
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 1- 238 (full-length) of jellyfish green fluorescent protein.
Application Notes
Immunofluorescence was preformed with this antibody in the brain of mice. Here it was bound to both yellow and green fluorescent protein (Kumar et al, 2015; PMID:25337655). This antibody was used for immunoprecipitation on Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Western blotting was preformed with this antibody on samples of Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Immunohistochemistry was preformed on zebrafish to visualize GFP (Mackenzie and Raible, 2012; PMID:23094043). Immunoprecipitation using this antibody was preformed on the sciatic nerves of rats infected with an adenovirus containing a GFP construct (Chang et al, 2014; PMID:25362471).
Antibody First Published
Thomas et al. A versatile method for cell-specific profiling of translated mRNAs in Drosophila PLoS One. 2012;7(7):e40276. PMID:22792260
Note on publication
Transgenic strains of Drosophila melanogaster expressing a GFP-tagged ribosomal protein, RpL10A, under the control of the UAS promoter to perform cell-type specific translatome profiling were created. The GFP::RpL10A fusion protein incorporates efficiently into ribosomes and polysomes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
