Cat. No.
MABL-162
Application
IP, PLA, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N93A/49.2
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 873-977 (cytoplasmic C-terminus) of rat GABABR1. GABABR1 is a component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins.
Alternative Names
N93A/49.2R; Gamma-aminobutyric acid type B receptor subunit 1; Gabbr1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1
UniProt
Q9Z0U4
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein of amino acids 873-977 (cytoplasmic C-terminus) of rat GABABR1.
Application Notes
This antibody was used in a proximity ligation assay on HEK293 cells transiently transfected using Lipofectamine 2000 with cDNA plasmids expressing GABABR1-eYFP, GABABR2 and GIRK2a (Li et al, 2020; PMID:32111696). Immunofluorescence was preformed on the hippocampal slices of P7 male Wistar rats using this antibody (Wright et al, 2017; PMID:28450542). An immunoprecipitation was preformed with this antibody on lysates from hippocampal slices of P7 male Wistar rats (Wright et al, 2017; PMID:28450542). Immunofluorescence was preformed on the brain of Tg(Gng3-GFP)- HK208Gsat and KI(Gng7-3-IRES-GFP) mice (Schwindinger et al, 2012; PMID:22207761).
Antibody First Published
Schwindinger et al. Synergistic roles for G-protein γ3 and γ7 subtypes in seizure susceptibility as revealed in double knock-out mice J Biol Chem. 2012 Mar 2;287(10):7121-33.
PMID:22207761
Note on publication
A gene targeting approach to determine whether the closely related γ(3) and γ(7) subunits can perform functionally interchangeable roles in mice was used.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
