Cat. No.
MABL-163
Application
IP, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N94/17
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 1-243 (full-length) of human FGF12a. This antibody cross-reacts with FGF12b/FHF1b. The antibody does not cross-react with FGF11/FHF3, FGF13/FHF2 or FGF14/FHF4. FGF12a is involved in nervous system development and function. Involved in the positive regulation of voltage-gated sodium channel activity. Promotes neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.
Alternative Names
N94/17R; Fibroblast growth factor 12; Fibroblast growth factor homologous factor 1; FHF-1; Myocyte-activating factor
UniProt
P61328
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein of amino acids 1- 243 (full-length) of human FGF12a.
Application Notes
This antibody is recommended for detection and analysis of FGF12 by immunohistochemistry and immunoprecipitation. For instance, the mouse version of this antibody was used to detect FGF12 by immunocytochemistry in COS cells expressing GFP-tagged FGF12b/FHF1b.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
Describes the generation of a library of recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
