Key features and details | |
Cat. No. | MABL-295 |
Name | Anti-17ß estradiol mAbs |
Clone No. | AFD-17E12E5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, RIA, WB, ELISA |
Species Reactivity | n/a |
Basic Information | |
Specificity | The antibody binds to 17ß estradiol with an ED50 of 76 pg/ml. |
Alternative Name | 17-beta-estradiol; E2; ethynyl estradiol; oestradiol |
UniProt | |
Immunogen | estradiol-6-ethyl methoxy carbonyl (EMC)–bovine serum albumin (BSA). |
Application Notes | The antibody binds specifically to 17β-estradiol, a mammalian estrogenic steroid hormone which is produced in the ovaries, placenta and testis. In women, the hormone plays an important role in the development of secondary sexual characteristics and maturation of long bones. The hormone is also responsible for the activation of certain oncogenes, and can be involved in the development of breast cancer and cancer of the uterine lining. Anti-estradiol antibodies can be useful in the detection of hormone concentrations in serum samples. This can be used therapeutically to monitor follicular maturation in cases of female infertility, and estradiol secreting tumours. |
Antibody First Published | Bettsworth F, Monnet C, Watelet B, Battail-Poirot N, Gilquin B, Jolivet M, Menez A, Arnaud M, Ducancel F. Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments. J Mol Recognit. 2001 Mar-Apr;14(2):99-109. PMID:11301480 |
Note on publication | Describes the role of monoclonal antibodies in measuring concentrations of steroid hormones in human serum samples, and the characterization of two anti-estradiol monoclonal antibodies for this use. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
