Key features and details | |
Cat. No. | MABL-3151 |
Name | Anti-TSH beta mAbs |
Clone No. | AFD- 14H9.E4.A4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, IF |
Species Reactivity | Cat, Dog, Horse, Sheep |
Basic Information | |
Specificity | This antibody recognises ovine, canine, feline and equine TSH beta which is a crucial hormone controlling thyroid functions. |
Alternative Name | Thyroid stimulating hormone b; Thyrotropin subunit beta; TSHB; Thyroid- stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; 14H9.E2 |
UniProt | P54828 |
Immunogen | The gene encoding the mature β subunit of canine thyroid stimulating hormone (cTSHβ) was cloned, sequenced and expressed in Escherichia coli and in Chinese hamster ovary (CHO) cells, and monoclonal antibodies against the recombinant cTSHβ, purified from E. coli, were generated. |
Application Notes | This antibody is capable of detecting TSH beta in a couple of important veterinary species. It may be used in the identification and analysis of TSH as well as TSH tests in veterinary medicine and research (Ferguson, 2007). For instance, 14H9.E4 linked to agarose beads was used to putify heterodimeric feline TSH (Rayalam et al., 2006). |
Antibody First Published | Rayalam et al. Expression and purification of feline thyrotropin (fTSH): immunological detection and bioactivity of heterodimeric and yoked glycoproteins. Domest Anim Endocrinol. 2006 Mar;30(3):185-202. Epub 2005 Aug 10. PMID:16125358 |
Note on publication | This article describes the original application of this antibody in an immunopurification procedure. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
