Key features and details | |
Cat. No. | MABL-3152 |
Name | Anti-Tu translation elongation factor mitochondrial mAbs |
Clone No. | AFD-Nb225 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human Tu translation elongation factor, mitochondrial (TUFM). This protein promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. These are chaperone-like properties involved in protein folding and overexpressed in colorectal carcinoma. |
Alternative Name | TUFM; Elongation factor Tu; EF-Tu; P43 |
UniProt | P49411 |
Immunogen | The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells. |
Application Notes | This antibody was used for identification of TUFM protein in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that TUFM expression was high in GBM compared to reference samples. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803). |
Antibody First Published | Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803 |
Note on publication | Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
