Cat. No.
MABL-345
Application
in vivo, IP, ELISA
Isotype
Engineer antibody
Species Reactivity
HIV-1
Clone No.
VH-12A
From
Recombinant Antibody
Specificity
The original antibody binds both acetylated and nonacetylated integrase.
Alternative Names
VH12; VH-12X; acetylated HIV-integrase
UniProt
Immunogen
The original antibody was isolated from a mouse naïve ScFv library by panning against acetylated HIV-integrase.
Application Notes
The original format of the antibody co-immunoprecipitated purified Ac-Integrase. The original format of the antibody reduced the viral infectivity on HeLa cells infected with an HIV virus. The original format of the antibody detected Ac-integrase by western blot analysis (US11137393B2).
Antibody First Published
Chirichella et al. Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies Nat Methods. 2017 Mar;14(3):279-282. doi: 10.1038/nmeth.4144. Epub 2017 Jan 16. PMID:28092690
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
