Cat. No.
MABL-398
Application
immunoblot, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BA7.1
From
Recombinant Antibody
Specificity
The antibody is specific for the 3,5-diaminopyrazinyl moiety of amiloride. Analogs with a benzyl on the 5-positition of the pyrazine ring or with the subsitution of an iodo for the chloro group in the 6- position of the ring don't bind. An analog with a benzyl substituent on a terminal nitrogen of the guanidinium group has an affinity for the antibody similar to amiloride.
Alternative Names
UniProt
Immunogen
The antibody was raised by immunising BALB/c mice with an intraperitoneal injection of an amiloride analogue coupled to BSA.
Application Notes
The antibody has been characterised using immunoblot analysis (Lin et al. 1994) and ELISA.
Antibody First Published
Kleyman et al. Distinct epitopes on amiloride Am. J. Physiol. 257 (Cell C1135-C1141, 1989 PMID:2481976
Note on publication
Describes the generation of polyclonal and monoclonal antibodies for amiloride
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
