Cat. No.
MABL-407
Application
ICC, Block, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Rabbit, Human
Clone No.
26-2F
From
Recombinant Antibody
Specificity
Binds to human angiogenin with a Kd of 1.6 nM. Minimal epitope is W89 and fragment 38-41 (PCKD).
Alternative Names
ANG; Ribonuclease 5; RNase 5; ALS9; HEL168; RAA1
UniProt
P03950
Immunogen
Human angiogenin.
Application Notes
The antibody binds specifically to angiogenin, a member of of the ribonuclease superfamily of proteins, which interacts with actin on the surface of endothelial and smooth muscle cells, inducing neovascularization. Due to its angiogenic activity, angiogenesis plays a key role in the growth and invasiveness of tumour cells, and therefore neutralising monoclonal antibodies such as 26-2F are important anticancer drugs. The monoclonal antibody 26-2F is not directly cytotoxic to tumour cells in vitro, however has been shown to have antagonistic effects in human breast cancer xenografts in athymic mice.
Antibody First Published
Fett et al. A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angionegnic activities and localization of the antigenic epitope. Biochemistry 1994; 33(18) 5421-5427 PMID 7514035
Note on publication
Describes the generation of a monoclonal antibody against human angiogenin and epitope mapping.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
