Cat. No.
MABL-552
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU20a
From
Recombinant Antibody
Specificity
This antibody recognises bromodeoxyuridine (BrdU) which is an analogue of thymidine.
Alternative Names
Bromodeoxyuridine; 5-bromo-2'-deoxyuridine; BUdR; broxuridine; BrdUrd
UniProt
Immunogen
This antibody was raised by immunising mice with bromodeoxyuridine conjugated to BSA.
Application Notes
Bu20a works well in immunohistochemistry to identify cells in tissue sections or cytospin preparations that have incorporated BrdU. It is also useful to identify cells in S phase during FACS analysis.
Antibody First Published
Magaud et al. Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37(10):1517-27 PMID:2476478
Note on publication
This article describes the generation and characterisation of BU20a antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
