Cat. No.
MABL-553
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
4E 5
From
Recombinant Antibody
Specificity
mAb 4E5 specifically recognises alpha-Brg1
Alternative Names
SMARCA4; alpha-BRG1; BAF190; BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4
UniProt
P51532
Immunogen
Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5
Application Notes
Antibody First Published
Ohkawa et al. Production of a Rat Monoclonal Antibody Against Brg1 HYBRIDOMA Volume 28, Number 6, 2009 PMID:20025509
Note on publication
Describes the role of BRG1 in the SWI/SNF complex as a DNA-dependent ATPase for chromatin remodelling, and mentions the need for a monoclonal antibody to distinguish BRG1 from BRM, which are highly similar.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
