Cat. No.
MABL-554
Application
crystallization, ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
BAG2
From
Recombinant Antibody
Specificity
The antibody is specific for BRIL.
Alternative Names
Soluble cytochrome b562; Cytochrome b-562
UniProt
P0ABE7
Immunogen
The original antibody was isolated from a semi-synthetic phage display library of humanized Fab fragments, by panning against BRIL.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The crystal structure of the antibody in complex with BRIL was determined by cryoEM (Mukherjee et al., 2020; PMID: 32221310).
Antibody First Published
Mukherjee et al., Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins. Nat Commun. 2020 Mar 27;11(1):1598. doi: 10.1038/s41467-020-15363-0. PMID:32221310
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
