Cat. No.
MABL-555
Application
ICC, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
IIB5
From
Recombinant Antibody
Specificity
The original antibody reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA. Further, the antibody is known to cross-react with Iododeoxyuridine (IdU).
Alternative Names
BrdU
UniProt
Immunogen
The original antibody was generated by immunizing a BALB/c mouse intraperitoneally with BrdU conjugated to Bovine Serum albumin.
Application Notes
The binding characteristics of the antibody was determined in a radioimmunoassay using 3H-BrdUrd as a ligand, and the affinity constant was found to be 3.1 x 10^7 L/mol (Schutte et al., 1987; PMID: 3113895). This antibody was used for immunohistochemistry for the detection of bromodeoxyuridine (van Engeland et al., 1997; PMID: 9299167).
Antibody First Published
Schutte et al. An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry Cytometry. 1987 Jul;8(4):372-6. doi: 10.1002/cyto.990080405. PMID:3113895
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
