Cat. No.
MABL-560
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10D11
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 1-175 of human BTK. It is known to bind to a 77-kD version of BTK. BTK is a non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling.
Alternative Names
Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
UniProt
Q06187
Immunogen
This antibody was raised by immunizing a mouse with the PH domain (amino acids 1-175) of human BTK.
Application Notes
A western blot on bkt proteins using the mouse version of this antibody has proven the specificity of this clone. The antibody recognized a 77-kD BTK. Furthermore the mouse version of this antibody allows for the immunoprecipitation of the btk protein (Bykowsky et al, 1996; pmid:8644706).
Antibody First Published
Bykowsky et al. Discordant phenotype in siblings with X-linked agammaglobulinemia Am J Hum Genet. 1996 Mar;58(3):477-83. PMID:8644706
Note on publication
The current study is the first description of two male siblings with identical T134 --> C mutations in the translation initiation ATG of Btk who have different clinical phenotypes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
