Cat. No.
MABL-563
Application
IP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
11D8
From
Recombinant Antibody
Specificity
11D8 binds to both native and denatured human butyrylcholinesterase (HuBChE) with KD ~0.26 nM (determined by ELISA - 0.9 nM by SPR). Binding of 11D8 excludes binding of mAb B2 18-5, suggesting tht that bind to the same/similar epitope on huBChE. The antibody also binds to human albumin, which can compete with huBChE for binding. huBChE in plasma is a serine esterase that catalyzes the hydrolysis of esters including aspirin, cocaine, heroin, and octanoyl ghrelin.
Alternative Names
BChE
UniProt
P06276
Immunogen
11D8 wsa prepared by immunizing BChE -/- mice with sugar-diminished full-length recombinant human BChE (Hrabovska et al. 2010).
Application Notes
The percent active HuBChE bound to 11D8 in ELISAS is highest when the volume of plasma was lowest. 11D8 also binds to human albumin and so 11D8 is less suitable for immunopurification of huBChE from plasma compared to e.g. B2 18-5.
Antibody First Published
Peng et al. Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads：KD values, binding pairs, and amino acid sequences. Chem Biol Interact. 2015 Oct 5;240：336-45 PMID：26343001
Note on publication
Describes the characterization and comparison of 5 monoclonal antibodies for their ability to purify huBChE on Dynabeads.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
