Cat. No.
MABL-564
Application
in vivo, IP, therapeutic, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
520C9
From
Recombinant Antibody
Specificity
The antibody binds an approximately 200 kD protein found in cancerous breast tissue which has been identified as c-erbB-2.
Alternative Names
Breast cancer cells; CD340; Receptor tyrosine-protein kinase erbB-2; Neu; HER2; MLN19; Metastatic lymph node gene 19 protein; p185erbB2
UniProt
P04626
Immunogen
The original antibody was generated by immunizing BALB/c mice with breast cancer cell lines.
Application Notes
The antibody stained breast tumors and breast cancer lines (Ring et al., 1989; PMID： 2470501). The antibody was employed in immunocytochemical assay to identify breast tumor cells (Franklin et al., 1996; PMID： 8932871). Fab fragment of the antibody was generated and tested for in vivo efficacy in the MX-1human breast tumor cell, where it showed a significant decrease in tumor volume (US5169774A). Immunoprecipitation tests on the antibody indicated that it bound to a monomeric c.a.210,000 Da protein found in cancerous breast tissue with high association constant (US6054561A). The antibody was further evaluated for range breast cancer recognition by cell line immunofluorescence assays. The antibody showed positive results on SKBr3, BT483, CAMA1 (US5629197A). The antibody was employed in bi-specific and tri-specific antibody development to detect c-erbB2 expression and breast cancer treatment (Press et al. 2005; PMID： 16166438). Furthermore, it was employed in developing tri-functional antibody ertumaxomab for breast cancer patients (Jager et al. 2009; PMID：19435924).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
