Cat. No.
MABL-569
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2A1
From
Recombinant Antibody
Specificity
This antibody binds the 2e loop of the human C3a receptor, amino acid residues 185-199 and 218–226 (TNDHPWTVP).
Alternative Names
C3AR1; C3AR; C3a-R; Complement component receptor 1; C3a anaphylatoxin chemotactic receptor
UniProt
Q16581
Immunogen
The original antibody was generated by immunizing BALB/c mice with second extracellular loop of the C3aR (amino acid residues 173–332) fused to glutathione-S-transferase (GST). A combinatorial phage library was generated expressing scFv antibodies and panned against second extracellular loop of the C3aR, fused to the maltose binding protein as antigen.
Application Notes
The original scFv antibody binds to the 2e loop of the human C3a receptor in an ELISA. Human C3a receptor is a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release. This antibody was able to bind RBL-2H3 cells stably expressing the C3aR and to the human mast cell line HMC-1 in a flow cytometric assay. This antibody is not capable of blocking the interaction between C3a and C3aR (PMID： 11373092).
Antibody First Published
Hawlisch et al. Site-specific anti-C3a receptor single-chain antibodies selected by differential panning on cellulose sheets. Analytica Biochemistry 2001; 293(1)：142-145.PMID：11373092
Note on publication
Describes the selection of scFv fragments with specificity for the C3a receptor from phage-display libraries.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
