Cat. No.
MABL-571
Application
WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7C12
From
Recombinant Antibody
Specificity
This antibody is specific for human C3b. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Alternative Names
Complement C3; C3; C3 and PZP-like alpha-2-macroglobulin domain- containing protein 1
UniProt
P01024
Immunogen
The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.
Application Notes
To prepare and characterize this antibody, an ELISA on iC3 protein was performed using the mouse version of this antibody (Tosic et al, 1989; pmid：2786912). An immunoblot was performed on control, early AD, and AD patient brain tissues using the mouse version of this antibody to determine the link between the complement system and Alzheimer’s disease (Wu et al, 2019; pmid：31433986). To study the deposition of the complement protein fragment C3b and its breakdown products on CD20-positive cells, flow cytometry was performed on Raji or ARH-77 cells using the mouse version of this antibody.
Antibody First Published
Wu et al. Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose J Immunol Methods. 1989 Jun 21;120(2)：241-9. PMID：2786912
Note on publication
Four monoclonal antibodies were prepared and characterized to human C3b of high specificity and affinity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
