Cat. No.
MABL-577
Application
Blocking, Functional Studies, IP, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Cow, Ferret, Rabbit, Human
Clone No.
S5/1
From
Recombinant Antibody
Specificity
S5/1 antibody recognizes the C5aR (CD88) protein, a 43 kDa receptor of C5a component of the complement cascade.
Alternative Names
CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR
UniProt
P21730
Immunogen
This antibody was raised by immunizing mice with a synthetic peptide corresponding to C5a- R; amino acids： 1-31 (N terminal).
Application Notes
S5/1 antibody inhibits C5a binding to its receptor C5aR (Oppermann et al., 1993). Furthermore, this antibody has been widely applied in the detection and analysis of C5aR via various methods. For instance, it was used in a flow cytometric study on peripheral blood lymphocytes where it was shown to bind to monocytes and granulocytes (Oppermann et al., 1993). It was also utilized in the Western blot experiments comparing C5aR expression in different groups of mice (Panayiotou et al., 2017). S5/1 was also successfull in labelling C5aR in the paraffin-embedded immunohistochemical samples of umbilical arteries (Fortin et al., 2002).
Antibody First Published
Oppermann et al. Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain. J Immunol. 1993 Oct 1;151(7)：3785-94. PMID：8376805
Note on publication
This article describes the generation and characterization of the S5/1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
