Cat. No.
MABL-578
Application
crystallization, MHA, RIA, SPR, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1116-NS-19-9
From
Recombinant Antibody
Specificity
This antibody is specific for human colorectal carcinoma cells.
Alternative Names
CD43; Colorectal carcinoma; CA19-9; sialyl Lewis A; sLe A; 1116-56-2; 1116NS19.9; 1116-NS-19–9
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with human colorectal carcinoma cell line SW 1116.
Application Notes
The original version of this antibody was used in a radioimmunoassay (RIA), a mixed Hemadsorption Assay (MHA), and an immunofluorescence (IF) assays to validate its specificity for human colorectal carcinoma cells. It was also tested on fresh tumor tissue from colorectal carcinoma patients, where it was found to bind selectively to tumor cells, but not normal intestinal tissue, further confirming its specificity for colorectal carcinoma (Herlyn et al., 1979; PMID： 286328). This antibody was crystallized with and without its target antigen. Furthermore, in a surface plasmon resonance (SPR) assay a KD of 14.7 µM was measured, and the antigen recognition of an scFv version of this antibody was measured via flow cytometry (FC) (Borenstein-Katz et al., 2021; PMID： 34119488). The use of this antibody in IHC was successfully demonstrated in a doctoral dissertation (Khilji, 2023).
Antibody First Published
Herlyn et al., Colorectal carcinoma-specific antigen： detection by means of monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3)：1438-42. PMID：286328
Note on publication
The original publication describes the development of monoclonal antibodies that specifically bind to human colorectal carcinoma cells, distinguishing them from normal colonic mucosa and other human cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
