Cat. No.
MABL-579
Application
ICC, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
121SLE
From
Recombinant Antibody
Specificity
This antibody is specific for CA19-9.
Alternative Names
121 SLE; sialyl Lewis a
UniProt
Immunogen
The original antibody was raised by immunizing a mouse with ten precipitin lines obtained after immunodiffusion using monoclonal antibody NS 19-9 and mucins isolated from an ovarian cyst of a OLe (a+b-) patient.
Application Notes
The original version of this antibody was used for immunohistochemistry on the normal gastrointestinal mucosa of Le-positive individuals (Herrero-Zabaleta et al., 1987; PMID：2444292), and on endometrial tissue specimens obtained in different phases of the menstrual cycle (Hey and Aplin., 1996; PMID：8910004). This antibody was used for an ELISA on carbohydrate proteins (Manimala et al., 2007; PMID：17483136), and on MUC1 samples (Hey and Aplin., 1996; PMID：8910004). Furthermore this antibody was used for immunocytochemistry on HT 29 tumour cells (Köhler et al., 2009; PMID：20010946). The original version of this antibody was used for flow cytometry on ovarian cancer cell line SW626 and SCLC cell line DMS79 (Ragupathi et al., 2009; PMID：19190907).
Antibody First Published
Herrero-Zabaleta et al. Monoclonal antibody against sialylated Lewis(a) antigen Bull Cancer. 1987;74(4):387-96. PMID:2444292
Note on publication
The original paper isolated hybridoma cells, which secreted monoclonal antibody (MAb) 121 SLE.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
