Cat. No.
MABL-584
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
VSA2
From
Recombinant Antibody
Specificity
This antibody binds caffeine and has low affinity for compounds like theophylline, theobromine and paraxanthine. Caffeine is a central nervous system (CNS) stimulant of the methylxanthine class. The best-known source of caffeine is the coffee bean, the seed of the Coffea plant. It is present in drinks like coffee, tea and colas.
Alternative Names
C8H10N4O2; guaranine; methyltheobromine; 1 3 7-Trimethylxanthine; 7- methyltheophylline; theine theophylline; theobromine; paraxanthine; CHEBI:27732; α-Caff-A
UniProt
Immunogen
The original antibody was generated by immunizing camelid species (llama and camel)) with caffeine-KLH conjugate. The peripheral blood lymphocytes from the camels was used to generate VHH phage display libraries and the particular clones was isolated by screening the library against caffeine-BSA conjugate.
Application Notes
This antibody is highly heat stable and is shown to recover its reactivity after exposure to temperatures up to 90 degrees C. This antibody was able to bind caffeine at 70 degrees C. The binding reactivity of this antibody for caffeine and other compounds like theophylline, theobromine and paraxanthine was determined using ELISA (PMID: 16808459; 19560409; US7615218). A competition caffeine ELISA was developed for the measurement of caffeine content in beverages like coffee, Coca-Cola and Diet Coke. This antibody is capable of removing caffeine from coffee and furthermore can remove caffeine from even “decaffeinated” coffee (PMID: 16808459; US7615218).
Antibody First Published
Ladenson et al. Isolation and characterization of a thermally stable recombinant anti-caffeine heavy-chain antibody fragment. Anal Chem. 2006 Jul 1;78(13):4501-8. PMID:16808459
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
