Cat. No.
MABL-588
Application
WB
Isotype
Engineer antibody
Species Reactivity
Cow
Clone No.
3B2-A2-E5
From
Recombinant Antibody
Specificity
This antibody is specific for calf thymus ATPase A and recognizes proteins containing both a polynucleotide binding site and a nucleotide binding site. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA.
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
UniProt
Q9TTA5
Immunogen
The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Application Notes
The mouse version of this antibody was used for a western blot on calf DNA- dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114).
Antibody First Published
Mesner et al. DNA-Dependent Adenosinetriphosphatase A Immunoaffinity Purification and Characterization of Immunological Reagents-1 Biochemistry. 1993 Aug 3;32(30):7772-8. PMID:8394114
Note on publication
An immunoaffinity purification of DNA-dependent ATPase A from fetal calf thymus is described.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
